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Ekmeklik buğdayda (Triticum aestivum L.) Rus buğday afidine dayanıklılık geninin ayrıntılı genetik haritalaması

Yıl 2021, Cilt: 58 Sayı: 4, 523 - 532, 21.12.2021

Meltem Evirgan Deniz İştipliler Muzaffer Tosun Hülya Yılmaz Temel Ferit Turanlı Fatma Aykut Tonk

https://doi.org/10.20289/zfdergi.836585

Öz

Amaç: Bu çalışmanın amacı Rus buğday afidine dayanıklılık sağlayan Dn100695 geninin bulunduğu 7D kromozomunu SSR markörleriyle ayrıntılı bir şekilde taramak ve dayanıklılık genini çevreleyen yeni SSR markörlerini belirlemektir.
Materyal ve Yöntem: Basribey x IG 100695 melezinin 190 bireyinden oluşan F2 populasyonu haritalama amacıyla kullanılmıştır. Haritalama populasyonu daha önceki markörlere ilave olarak 7D kromozomunda bulunan 20 yeni SSR markörleri ile taranmıştır. Zararlının populasyondaki kloroz, kıvrılma ve afit yoğunluğu özellikleri fenotipik veriler olarak ele alınmıştır.
Araştırma Bulguları ve Sonuç: Çalışma sonucunda 85 polimorfik markör içerisinden 46’sı toplamda 1226.9 cM’lık bir genom bölgesini haritalandırmış ve 14 farklı bağlantı grubu oluşturmuştur. 7D kromozomu üzerinde dayanıklılık genini çevreleyen özgün bir SSR markörü (GWM121) belirlenmiş ve Dn100695 genini her iki yönden çevreleyen markörler haritalanmıştır. Ayrıca markör regresyon analizi sonucunda Rus buğday afidi dayanıklılığına katkı sağlama potansiyeli bulunan SSR markörleri de saptanmıştır.

Anahtar Kelimeler

Buğday Rus buğday afidi Diuraphis noxia SSR Ayrıntılı haritalama

Destekleyen Kurum

TÜBİTAK ve Ege Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Proje Numarası

117 O 049 ve 2015-ZRF-050

Teşekkür

Bu çalışma TÜBİTAK (Proje NO:117 O 049) ve Ege Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü (Proje NO: 2015-ZRF-050) tarafından desteklenmiştir.

Kaynakça

  • Castro, A.M., A. Vasicek, M. Manifiesto, D.O. Giménez, M.S. Tacaliti, O. Dobrovolskaya, M.S. Röder, J.W. Snape and A. Börner. 2005. Mapping antixenosis genes on chromosome 6A of wheat to greenbug and to a new biotype of Russian wheat aphid. Plant Breeding, 124(3):229-233.
  • Cockrell, D.M., T.L. Randolph, C.B. Walker, R. Sitz, S.D. Haley and F.B. Peairs. 2020. Damage and yield response of wheat lines with Dn4 and Dn7 genes resistant to Russian wheat aphid biotype RWA2. Southwestern Entomologist, 45(2):369-380.
  • Çakir, M., J. Vitou, D. Kollehn, W. Lawson, H. Ilbi, S. Haley, F. Peairs, D. Mornhinweg, M. Bohssini, F. Ogbonnaya, J. Lage, V. Tolmay, J. Malinga, O. Edwards, M. Christopher, A.M. Castro, J. Franckowiak, H. Kuchel, B. Jacobs, I. Barclay, and J. Sheppard. 2009. “A Global Effort in Breeding for Resistance to Russian Wheat Aphid in Wheat and Barley”, Prooc, of 19th, International Triticeae Mapping Initiative–3rd COST Tritigen, Clermont-Ferrand- France, p. 38.
  • Doyle, J.J., J.L. Doyle. 1987. A Rapid DNA Isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19:11-15.
  • Ennahli, S., M. El Bouhssini, S. Grando, R. Anathakrishnan, T. Niide, L. Starkus, S. Starkey and C.M. Smith. 2009. Comparison of categories of resistance in wheat and barley genotypes against biotype 2 of the Russian wheat aphid, Diuraphis noxia (Kurdjumov). Arthropod-Plant Interactions, 3:45-53.
  • Fouche, A., R.L. Verhoeven, P.H. Hewitt, M.C. Walters, C.F. Kriel and J. De Jager. 1984. Russian aphid (Diuraphis noxia) feding damage on wheat, related cereals and a Bromus grass species. Progress in Russian wheat aphid (Diuraphis noxia Mordw.) research in the Republic of South Africa. M. C. Walters. Bloemfontein, Technical Communication Department of Agriculture Republic of South Africa, 191, 22-23.
  • Girma, M., G.E. Wilde and T.L. Harvey. 1993. Russian wheat aphid (Homoptera: Aphididae) affects yield and quality of wheat. Journal of Economic Entomology, 86:594-601.
  • GrainGenes, 2017: GrainGenes 2.0: A Database for Triticeae and Avena. USDA-ARS, Washington, DC. Available at: http://wheat.pw.usda.gov/ cgi-bin/graingenes/browse.cgi?class=marker Erişim Mayıs 2017.
  • Haley, S.D., F.B. Pearis, C.B. Walker, J.B. Rudolph, T.L. Randolph. 2004. Occurrence of a New Russian Wheat Aphid Biotype in Colorado. Crop Science, 44:1589-92.
  • Heyns, I., E. Groenewald, F. Marais, F. Du Toit and V Tolmay. 2006. Chromosomal location of the Russian wheat aphid resistance gene, Dn5. Crop Science, 46(2):630-636.
  • Joukhadar, R., M. El-Bouhssini, A. Jighly and F.C. Ogbonnaya. 2013. Genome-wide association mapping for five major pest resistances in wheat. Molecular Breeding, 32(4):943-960.
  • Keller, B., C. Feuillet and M. Messmer. 2000. Genetics of disease resistance. In Mechanisms of Resistance to Plant Diseases. Springer, Dordrecht, pp 101-160.
  • Kosambi, D.D. 1944. The estimation of map distancesfrom recombination values. Annals of Eugenics, 12:172-5.
  • Kumar, N., P.L. Kulwal, A. Gaur, A.K. Tyagi, J.P. Khurana, P. Khurana, H.S. Balyan and P.K. Gupta. 2006. QTL analysis for grain weight in common wheat. Euphytica, 151(2): 135-144.
  • Landjeva, S., V. Korzun and A. Börner. 2007. Molecular markers: Actual and potential contributions to wheat genome characterization and breeding. Euphytica, 156(3):271-296.
  • Lapitan, N.L.V., J.H. Peng and V. Sharma. 2007. A high-density map and PCR markers for Russian wheat aphid resistance gene Dn7 on chromosome 1RS/1BL. Crop Science, 47:811-820.
  • Liu, S., M.D. Hall, C.A. Griffey and A.L. McKendry. 2009. Meta-Analysis of QTL associated with fusarium head blight resistance in wheat. Crop Science, 49(6):1955–1968.
  • Liu, X.M., C.M. Smith and B.S. Gill. 2002. Identification of microsatellite markers linked to Russian wheat aphid resistance genes Dn4 and Dn6. Theoretical and Applied Genetics, 104(6-7):1042-1048.
  • Liu, X.M., C.M. Smith, B.R. Friebe and B.S. Gill. 2005. Molecular mapping and allelic relationships of Russian wheat aphid-resistance genes. Crop Science, 45(6):2273-2280.
  • Liu, X.M., C.M. Smith, B.S. Gill and V. Tolmay. 2001. Microsatellite markers linked to six Russian wheat aphid resistance genes in wheat. Theoretical and Applied Genetics, 102(4):504-510.
  • Malinga, J.N., M.G. Kinyua, A.W. Kamau, J.K. Wanjama, J.O. Awalla and R.S. Pathak. 2007. Biotypic and genetic variation within tropical populations of Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Homoptera: Aphididae) in Kenya. Journal of Entomology, 4(5):350- 61.
  • Marone, D., G. Laidò, A. Gadaleta, P. Colasuonno, D.B.M. Ficco, A. Giancaspro, S. Giove, G. Panio, M.A. Russo, P. De Vita, L. Cattivelli, R. Papa, A. Blanco, A.M. Mastrangelo. 2012. A high-density consensus map of A and B wheat genomes. Theoretical and Applied Genetics , 125(8):1619-1638.
  • Miller, C.A., A. Altinkut and N.L.V. Lapitan. 2001. A microsatellite marker for tagging Dn2, a wheat gene conferring resistance to the Russian wheat aphid. Crop Science, 41(5):1584-1589.
  • Quick, J. S., K.K. Nkongolo, W. Meyer, F.B. Peairs and B. Weaver. 1991. Russian wheat aphid reaction and agronomic and quality traits of a resistant wheat. Crop Science, 31:50-53.
  • Somers, D.J., P. Isaac and K. Edwards. 2004. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 109(6):1105-1114.
  • Tolmay, V.L., S.L. Sydenham, T.N. Sikhakhane, B.N. Nhlapho and T.J. Tsilo. 2020. Elusive diagnostic markers for Russian wheat aphid resistance in bread wheat: Deliberating and reviewing the status quo. International Journal of Molecular Sciences, 21(21):8271.
  • Tonk, F.A., D. İştipliler ve M. Tosun. 2017. Bazı ekmeklik buğday (Triticum aestivum L.) genotiplerinde özellikler arası ilişkiler ve path analizi. Ege Üniversitesi Ziraat Fakültesi Dergisi, 54(1):85-89.
  • Tonk, F.A., D. İştipliler, M. Tosun, F. Turanli, H. İlbi and M. Çakir. 2016. Genetic mapping and inheritance of Russian wheat aphid resistance gene in accession IG 100695. Plant Breeding, 135(1):21–25.
  • Turanlı, F., M. Tosun, E. İlker, A.H. Sekmen ve F.A. Tonk. 2014. Rus Buğday Afidi (Diuraphis noxia Homoptera;Aphididae)’ne Dayanıklı Buğday Çeşitlerimizin ve Dayanıklılık Mekanizmalarının Saptanması’. TÜBİTAK, Proje no: 110 0 636, Ankara.
  • Valdez, V.A., P.F. Byrne, N.L.V. Lapitan, F.B. Peairs, A. Bernardo, G. Bai and S.D. Haley. 2012. Inheritance and genetic mapping of Russian wheat aphid resistance in Iranian wheat landrace accession PI 626580. Crop Science, 52(2):676-682.
  • Van Ooijen, J.W. 1999. LOD significance thresholds for QTL analysis in experimental populations of diploid species. Heredity, 83(5):613-624.
  • Van Ooijen, J.W. 2006. JoinMap 4, Software for the calculation of genetic linkage maps in experimental populations. Kyazma BV, Wageningen, the Netherlands.
  • Van Ooijen, J.W. 2009. MapQTL 6, software for the mapping of quantitative trait loci in experimental populations of diploid species. Kyazma BW, Wageningen, the Netherlands.
  • Webster, J.A., K.J. Starks and R.L. Burton. 1987. Plant resistance studies with Diuraphis noxia (Homoptera: Aphididae), a new United States wheat pest. Journal of Economic Entomology, 80:944-949.

Fine Mapping of Russian Wheat Aphid Resistance Gene in Bread Wheat (Triticum aestivum L.)

Yıl 2021, Cilt: 58 Sayı: 4, 523 - 532, 21.12.2021

Meltem Evirgan Deniz İştipliler Muzaffer Tosun Hülya Yılmaz Temel Ferit Turanlı Fatma Aykut Tonk

https://doi.org/10.20289/zfdergi.836585

Öz

Objective: The aim of this study was to screen the chromosome 7D with SSR markers and to identify unique markers surrounding the Russian wheat aphid resistance gene Dn100695.
Material and methods: The F2 population consisting of 190 individuals of the Basribey x IG 100695 cross was used for mapping. The mapping population was screened with 20 SSR markers located on the 7D chromosome in addition to the previous markers. Chlorosis, rolling and aphid density characteristics of the pest in the population were considered as phenotypic data.
Results and conclusion: As a result of the study, 46 of the 85 polymorphic markers mapped a genome region of 1226.9 cM in total and formed 14 different linkage groups. A unique SSR marker (GWM121) flanking the resistance gene was identified on the 7D chromosome, and markers flanking the Dn100695 gene from both directions were mapped. Additionally, as a result of the marker regression analysis, SSR markers that have the potential to contribute to Russian wheat aphid resistance were determined.

Anahtar Kelimeler

Wheat Russian wheat aphid Diuraphis noxia SSR Fine mapping

Proje Numarası

117 O 049 ve 2015-ZRF-050

Kaynakça

  • Castro, A.M., A. Vasicek, M. Manifiesto, D.O. Giménez, M.S. Tacaliti, O. Dobrovolskaya, M.S. Röder, J.W. Snape and A. Börner. 2005. Mapping antixenosis genes on chromosome 6A of wheat to greenbug and to a new biotype of Russian wheat aphid. Plant Breeding, 124(3):229-233.
  • Cockrell, D.M., T.L. Randolph, C.B. Walker, R. Sitz, S.D. Haley and F.B. Peairs. 2020. Damage and yield response of wheat lines with Dn4 and Dn7 genes resistant to Russian wheat aphid biotype RWA2. Southwestern Entomologist, 45(2):369-380.
  • Çakir, M., J. Vitou, D. Kollehn, W. Lawson, H. Ilbi, S. Haley, F. Peairs, D. Mornhinweg, M. Bohssini, F. Ogbonnaya, J. Lage, V. Tolmay, J. Malinga, O. Edwards, M. Christopher, A.M. Castro, J. Franckowiak, H. Kuchel, B. Jacobs, I. Barclay, and J. Sheppard. 2009. “A Global Effort in Breeding for Resistance to Russian Wheat Aphid in Wheat and Barley”, Prooc, of 19th, International Triticeae Mapping Initiative–3rd COST Tritigen, Clermont-Ferrand- France, p. 38.
  • Doyle, J.J., J.L. Doyle. 1987. A Rapid DNA Isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19:11-15.
  • Ennahli, S., M. El Bouhssini, S. Grando, R. Anathakrishnan, T. Niide, L. Starkus, S. Starkey and C.M. Smith. 2009. Comparison of categories of resistance in wheat and barley genotypes against biotype 2 of the Russian wheat aphid, Diuraphis noxia (Kurdjumov). Arthropod-Plant Interactions, 3:45-53.
  • Fouche, A., R.L. Verhoeven, P.H. Hewitt, M.C. Walters, C.F. Kriel and J. De Jager. 1984. Russian aphid (Diuraphis noxia) feding damage on wheat, related cereals and a Bromus grass species. Progress in Russian wheat aphid (Diuraphis noxia Mordw.) research in the Republic of South Africa. M. C. Walters. Bloemfontein, Technical Communication Department of Agriculture Republic of South Africa, 191, 22-23.
  • Girma, M., G.E. Wilde and T.L. Harvey. 1993. Russian wheat aphid (Homoptera: Aphididae) affects yield and quality of wheat. Journal of Economic Entomology, 86:594-601.
  • GrainGenes, 2017: GrainGenes 2.0: A Database for Triticeae and Avena. USDA-ARS, Washington, DC. Available at: http://wheat.pw.usda.gov/ cgi-bin/graingenes/browse.cgi?class=marker Erişim Mayıs 2017.
  • Haley, S.D., F.B. Pearis, C.B. Walker, J.B. Rudolph, T.L. Randolph. 2004. Occurrence of a New Russian Wheat Aphid Biotype in Colorado. Crop Science, 44:1589-92.
  • Heyns, I., E. Groenewald, F. Marais, F. Du Toit and V Tolmay. 2006. Chromosomal location of the Russian wheat aphid resistance gene, Dn5. Crop Science, 46(2):630-636.
  • Joukhadar, R., M. El-Bouhssini, A. Jighly and F.C. Ogbonnaya. 2013. Genome-wide association mapping for five major pest resistances in wheat. Molecular Breeding, 32(4):943-960.
  • Keller, B., C. Feuillet and M. Messmer. 2000. Genetics of disease resistance. In Mechanisms of Resistance to Plant Diseases. Springer, Dordrecht, pp 101-160.
  • Kosambi, D.D. 1944. The estimation of map distancesfrom recombination values. Annals of Eugenics, 12:172-5.
  • Kumar, N., P.L. Kulwal, A. Gaur, A.K. Tyagi, J.P. Khurana, P. Khurana, H.S. Balyan and P.K. Gupta. 2006. QTL analysis for grain weight in common wheat. Euphytica, 151(2): 135-144.
  • Landjeva, S., V. Korzun and A. Börner. 2007. Molecular markers: Actual and potential contributions to wheat genome characterization and breeding. Euphytica, 156(3):271-296.
  • Lapitan, N.L.V., J.H. Peng and V. Sharma. 2007. A high-density map and PCR markers for Russian wheat aphid resistance gene Dn7 on chromosome 1RS/1BL. Crop Science, 47:811-820.
  • Liu, S., M.D. Hall, C.A. Griffey and A.L. McKendry. 2009. Meta-Analysis of QTL associated with fusarium head blight resistance in wheat. Crop Science, 49(6):1955–1968.
  • Liu, X.M., C.M. Smith and B.S. Gill. 2002. Identification of microsatellite markers linked to Russian wheat aphid resistance genes Dn4 and Dn6. Theoretical and Applied Genetics, 104(6-7):1042-1048.
  • Liu, X.M., C.M. Smith, B.R. Friebe and B.S. Gill. 2005. Molecular mapping and allelic relationships of Russian wheat aphid-resistance genes. Crop Science, 45(6):2273-2280.
  • Liu, X.M., C.M. Smith, B.S. Gill and V. Tolmay. 2001. Microsatellite markers linked to six Russian wheat aphid resistance genes in wheat. Theoretical and Applied Genetics, 102(4):504-510.
  • Malinga, J.N., M.G. Kinyua, A.W. Kamau, J.K. Wanjama, J.O. Awalla and R.S. Pathak. 2007. Biotypic and genetic variation within tropical populations of Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Homoptera: Aphididae) in Kenya. Journal of Entomology, 4(5):350- 61.
  • Marone, D., G. Laidò, A. Gadaleta, P. Colasuonno, D.B.M. Ficco, A. Giancaspro, S. Giove, G. Panio, M.A. Russo, P. De Vita, L. Cattivelli, R. Papa, A. Blanco, A.M. Mastrangelo. 2012. A high-density consensus map of A and B wheat genomes. Theoretical and Applied Genetics , 125(8):1619-1638.
  • Miller, C.A., A. Altinkut and N.L.V. Lapitan. 2001. A microsatellite marker for tagging Dn2, a wheat gene conferring resistance to the Russian wheat aphid. Crop Science, 41(5):1584-1589.
  • Quick, J. S., K.K. Nkongolo, W. Meyer, F.B. Peairs and B. Weaver. 1991. Russian wheat aphid reaction and agronomic and quality traits of a resistant wheat. Crop Science, 31:50-53.
  • Somers, D.J., P. Isaac and K. Edwards. 2004. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 109(6):1105-1114.
  • Tolmay, V.L., S.L. Sydenham, T.N. Sikhakhane, B.N. Nhlapho and T.J. Tsilo. 2020. Elusive diagnostic markers for Russian wheat aphid resistance in bread wheat: Deliberating and reviewing the status quo. International Journal of Molecular Sciences, 21(21):8271.
  • Tonk, F.A., D. İştipliler ve M. Tosun. 2017. Bazı ekmeklik buğday (Triticum aestivum L.) genotiplerinde özellikler arası ilişkiler ve path analizi. Ege Üniversitesi Ziraat Fakültesi Dergisi, 54(1):85-89.
  • Tonk, F.A., D. İştipliler, M. Tosun, F. Turanli, H. İlbi and M. Çakir. 2016. Genetic mapping and inheritance of Russian wheat aphid resistance gene in accession IG 100695. Plant Breeding, 135(1):21–25.
  • Turanlı, F., M. Tosun, E. İlker, A.H. Sekmen ve F.A. Tonk. 2014. Rus Buğday Afidi (Diuraphis noxia Homoptera;Aphididae)’ne Dayanıklı Buğday Çeşitlerimizin ve Dayanıklılık Mekanizmalarının Saptanması’. TÜBİTAK, Proje no: 110 0 636, Ankara.
  • Valdez, V.A., P.F. Byrne, N.L.V. Lapitan, F.B. Peairs, A. Bernardo, G. Bai and S.D. Haley. 2012. Inheritance and genetic mapping of Russian wheat aphid resistance in Iranian wheat landrace accession PI 626580. Crop Science, 52(2):676-682.
  • Van Ooijen, J.W. 1999. LOD significance thresholds for QTL analysis in experimental populations of diploid species. Heredity, 83(5):613-624.
  • Van Ooijen, J.W. 2006. JoinMap 4, Software for the calculation of genetic linkage maps in experimental populations. Kyazma BV, Wageningen, the Netherlands.
  • Van Ooijen, J.W. 2009. MapQTL 6, software for the mapping of quantitative trait loci in experimental populations of diploid species. Kyazma BW, Wageningen, the Netherlands.
  • Webster, J.A., K.J. Starks and R.L. Burton. 1987. Plant resistance studies with Diuraphis noxia (Homoptera: Aphididae), a new United States wheat pest. Journal of Economic Entomology, 80:944-949.
Toplam 34 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Meltem Evirgan Bu kişi benim 0000-0002-8916-5041

Deniz İştipliler 0000-0002-0887-1121

Muzaffer Tosun 0000-0001-7989-4737

Hülya Yılmaz Temel Bu kişi benim 0000-0003-1842-0145

Ferit Turanlı 0000-0003-1096-1756

Fatma Aykut Tonk 0000-0003-4557-5919

Proje Numarası 117 O 049 ve 2015-ZRF-050
Yayımlanma Tarihi 21 Aralık 2021
Gönderilme Tarihi 7 Aralık 2020
Kabul Tarihi 31 Mart 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 58 Sayı: 4

Kaynak Göster

APA Evirgan, M., İştipliler, D., Tosun, M., Yılmaz Temel, H., vd. (2021). Ekmeklik buğdayda (Triticum aestivum L.) Rus buğday afidine dayanıklılık geninin ayrıntılı genetik haritalaması. Ege Üniversitesi Ziraat Fakültesi Dergisi, 58(4), 523-532. https://doi.org/10.20289/zfdergi.836585
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