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The Preparation and Characterization of Electrospun PCL/PHBV Membranes as Wound Dressing Material

Yıl 2019, Cilt: 8 Sayı: 3, 1029 - 1044, 30.09.2019

İsmail Alper İşoğlu

https://doi.org/10.17798/bitlisfen.530767
Cited By: 1

Öz





















In this study, fibrous
membranes of Polycaprolactone (PCL), Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
(PHBV) and PCL/PHBV blends in different weight ratios (100:0, 50:50, 75:25,
0:100) were prepared using different solvents (chloroform (CHCl3),
1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP) and their blends) via electrospinning
technique as wound dressing materials. Structural, morphological, and thermal
characterizations of membranes were performed by Fourier Transform Infrared
Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM) and Differential
Scanning Calorimetry (DSC), respectively. Moreover, the water-uptake capacity
of the membranes were analyzed using absorption test. Following that, human
fibroblast cells were seeded on the selected membranes and in vitro cell viability and toxicity were monitored by MTT assay
for 24, 48 and 72th hours.  The
seeded cell proliferation on membranes was conducted for 36, 72 and 120th
hours and the results were examined by SEM. The results of this study relieved
that electrospun PCL/PHBV (75:25) blend membrane solved in HFIP can be used as
wound dressing materials.    

Anahtar Kelimeler

PCL PHBV Electrospinning Wound Dressing Material

Kaynakça

  • 1. Kuppan, P., Kirthanashri S.V., Dhakshinamoorthy S., Uma M.K., and Swaminathan S. 2011. Development of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Fibers for Skin Tissue Engineering: Effects of Topography, Mechanical, and Chemical Stimuli, Biomacromolecules, 12 (9): 3156–3165.
  • 2. Zonari, A., Cerqueira M.T., Novikoff, S., Goes A.M., Marques, A.P., Correlo, V.M. and Reis, R.L. 2014. Poly(hydroxybutyrate-co-hydroxyvalerate) Bilayer Skin Tissue Engineering Constructs with Improved Epidermal Rearrangement, Macromolecular Bioscience, 14 (7): 977–990.
  • 3. Du, L., Xu, H.Z., Li, T., Zhang, Y. and Zou, F.Y. 2017. Fabrication of Ascorbyl Palmitate Loaded Poly(caprolactone)/Silver Nanoparticle Embedded Poly(vinyl alcohol) Hybrid Nanofibre Mats as Active Wound Dressings: Via Dual-Spinneret Electrospinning, RSC Advances, 7 (50): 31310–31318.
  • 4. Augustine R., Anto Dominic E., Reju I., Kaimal B., Kalarikkal N., Thomas S. 2015. Electrospun Poly(e-caprolactone)-Based Skin Substitutes: In Vivo Evaluation of Wound Healing and The Mechanism of Cell Proliferation, Journal of Biomedical Materials Research Part B: Applied Biomaterials, 103 (7): 1445–1454.
  • 5. Sahana, T.G., Rekha, P.D. 2018. Biopolymers: Applications in Wound Healing and Skin Tissue Engineering, Molecular Biology Report, 45 (6): 2857-2867.
  • 6. Moura D., Souza M.T., Liverani L., Rella G., Luz G.M., Mano J.F., Boccaccini A.R. 2017. Development of A Bioactive Glass-Polymer Composite for Wound Healing Applications, Materials Science and Engineering C, 76: 224-232.
  • 7. Sundaramurthi D., Krishnan U.M., Sethuraman S. 2014. Epidermal Differentiation of Stem Cells on Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) Nanofibers, Annals of Biomedical Engineering, 42 (12): 2589–2599.
  • 8. Lee J.M., Chae T., Sheikh F.A., Ju H.W., Moon B.M., Park H.J., Park Y.R., Park C.H. 2016. Three Dimensional Poly(ε-caprolactone) and Silk Fibroin Nanocomposite Fibrous Matrix for artificial Dermis, Materials Science and Engineering: C, 68: 758–67.
  • 9. MacEwan M.R., Macewan S., Kovacs T.R., Batts J. 2017. What Makes the Optimal Wound Healing Material? A Review of Current Science and Introduction of a Synthetic Nanofabricated Wound Care Scaffold. 9 (10), 1736-1748.
  • 10. Biazar E. 2017. Application of Polymeric Nanofibers in Medical Designs, Part I: Skin and Eye, International Journal of Polymeric Materials and Polymeric Biomaterials, 66 (10): 521–531.
  • 11. Miguel S.P., Figueira D.R., Simões D., Ribeiro M.P., Coutinho P., Ferreira P., Correia I.J. 2018. Electrospun Polymeric Nanofibres as Wound Dressings: A Review, Colloids Surfaces B Biointerfaces, 169: 60–71.
  • 12. Lv F., Wang J., Xu P., Han Y., Ma H., Xu H., Chen S., Chang J., Ke Q., Liu M., Yi Z., Wu C. 2017. A Conducive Bioceramic/Polymer Composite Biomaterial for Diabetic Wound Healing, Acta Biomaterialia, 60: 128–143.
  • 13. Bölgen N., Menceloǧlu Y.Z., Acatay K., Vargel I., Pişkin E. 2005 In Vitro and In Vivo Degradation of Non-Woven Materials Made of Poly(Ε-Caprolactone) Nanofibers Prepared by Electrospinning Under Different Conditions. Journal of Biomaterials Science, Polymer Edition, 16 (12): 1537–1555.
  • 14. Rather H.A., Thakore R., Singh R., Jhala D., Singh S., Vasita R. Antioxidative Study of Cerium Oxide Nanoparticle Functionalised PCL-Gelatin Electrospun Fibers for Wound Healing Application, Bioactive Materials, 3 (2): 201–211.
  • 15. Yuan J., Geng J., Xing Z., Shim K-J., Han I., Kim J-C., Kang I-K., Shen J. 2015. Novel Wound Dressing Based on Nanofibrous PHBV–Keratin Mats, Journal of Tissue Engineering and Regenerative Medicine, 9: 1027–1035.
  • 16. Tohidi S., Ghaee A., Barzin J. 2016, Preparation and Characterization of Poly(lactic-co-glycolic acid)/Chitosan Electrospun Membrane Containing Amoxicillin-Loaded Halloysite Nanoclay, Polymers for Advanced Technologies, 27: 1020-1028.
  • 17. Dias J.R., Granja P.L., Bártolo P.J. 2016. Advances in Electrospun Skin Substitutes, Progress in Materials Science, 84: 314-334.
  • 18. Zhang W., Ronca S., Mele E. 2017. Electrospun Nanofibres Containing Antimicrobial Plant Extracts, Nanomaterials, 7 (2): 42-59.
  • 19. Patil J.V., Mali S.S., Kamble A.S., Hong C.K., Kim J.H., Patil P.S. 2017. Electrospinning: a Versatile Technique for Making of 1D Growth of Nanostructured Nanofibers and Its Applications: An Experimental Approach. Applied Surface Science, 423: 641–674.
  • 20. Chen S., Liu B., Carlson M.A., Gombart A.F., Reilly D.A., Xie J. 2017. Recent Advances in Electrospun Nanofibers for Wound Healing, Nanomedicine, 12: 1335-1352.
  • 21. Liu M., Duan X., Li Y., Yang D., Long Y. 2017. Electrospun Nano Fibers for Wound Healing, Materials Science and Engineering C, 76: 1413-1423.
  • 22. Mir M., Ali M.N., Barakullah A., Gulzar A., Arshad M, Fatima S., Asad M. 2018. Synthetic Polymeric Biomaterials for Wound Healing: A Review, Progress in Biomaterials, 7 (1): 1-21.
  • 23. Siddiqui N., Asawa S., Birru B., Baadhe R., Rao S. 2018. PCL-Based Composite Scaffold Matrices for Tissue Engineering Applications. Molecular Biotechnology, 60: 506–532.
  • 24. Thanh Tra N., Minh H.H., Nam T.M.H., Thien D.B.T., Hoai, N.T.T., Phuoc, T.V., Thai D.M., Hai, N.D., Toi, V.V., Hiep, N.T. 2018. Optimization and Characterization of Electrospun Polycaprolactone Coated with Gelatin-Silver Nanoparticles for Wound Healing Application, Materials Science & Engineering C, 91: 318-329.
  • 25. Martins A.F., Facchi S.P., da Câmara P.C.F., Camargo S.E.A., Camargo C.H.R., Popat K.C., Kipper M.J. 2018. Novel Poly(ε-Caprolactone)/Amino-Functionalized Tannin Electrospun Membranes as Scaffolds for Tissue Engineering, Journal of Colloid and Interface Science, 525: 21-30.
  • 26. Mutlu G., Calamak S., Ulubayram K., Guven E. 2018. Curcumin-Loaded Electrospun PHBV Nanofibers as Potential Wound-Dressing Material, Journal of Drug Delivery Science and Technology, 43: 185-193.
  • 27. Veleirinho B., Coelho D.S., Dias P.F., Maraschin M., Ribeiro-do-Valle R.M., Lopes-da-Silva J.A. 2012. Nanofibrous Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/Chitosan Scaffolds for Skin Regeneration. International Journal of Biological Macromolecules, 51 (4): 343-350.
  • 28. Shishatskaya E.I., Nikolaeva E.D., Vinogradova O.N., Volova T.G. 2016. Experimental Wound Dressings of Degradable PHA for Skin Defect Repair, Journal of Materials Science: Materials in Medicine, 27 (11): 165.
  • 29. Adeli-Sardou M., Yaghoobi M.M., Torkzadeh-Mahani M., Dodel M. 2019. Controlled Release of Lawsone from Polycaprolactone/Gelatin Electrospun Nano Fibers for Skin Tissue Regeneration, International Journal of Biological Macromolecules, 124: 478-491.
  • 30. Ehterami A., Salehi M., Farzamfar S., Vaez A., Samadian H., Sahrapeyma H., Mirzaii M., Ghorbani S., Goodarzi A. 2018. In Vitro and In Vivo Study of PCL/COLL Wound Dressing Loaded with Insulin-Chitosan Nanoparticles on Cutaneous Wound Healing in Rats Model, International Journal of Biological Macromolecules, 117: 601-609.
  • 31. İşoğlu İ.A., Demirkan C., Şeker M.G., Tuzlakoğlu K., İşoğlu S.D. 2018. Antibacterial Bilayered Skin Patches Made of HPMA and Quaternary Poly(4-Vinyl Pyridine). Fibers and Polymers, 19 (11): 2229-2236.
  • 32. K-Hasuwan P.R., Pavasant P., Supaphol P. 2011. Effect of the Surface Topography of Electrospun Poly(ε-caprolactone)/Poly(3-hydroxybuterate-co-3-hydroxyvalerate) Fibrous Substrates on Cultured Bone Cell Behavior. Langmuir. 27: 10938-10946.
  • 33. Malikmammadov E., Tanir T.E., Kiziltay A., Hasirci V., Hasirci N. 2018. PCL and PCL-Based Materials in Biomedical Applications, Journal of Biomaterials Science, Polymer Edition, 29 (7-9): 863-893.
  • 34. Del Gaudio C., Ercolani E., Nanni F., Bianco A. 2011. Assessment of Poly(ε-caprolactone)/Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Blends Processed by Solvent Casting and Electrospinning, Materials Science and Engineering A, 528 (3): 1764-1772.
  • 35. Chanda A., Adhikari J., Ghosh A., Roy S., Thomas S., Datta P., Saha P. 2018. Electrospun Chitosan/Polycaprolactone-Hyaluronic Acid Bilayered Scaffold for Potential Wound Healing Applications, International Journal of Biological Macromolecules, 116: 774-785.

Yara Örtü Malzemesi Olarak Elektroeğrilmiş PCL/PHBV Membranların Hazırlanması ve Karakterizasyonu

Yıl 2019, Cilt: 8 Sayı: 3, 1029 - 1044, 30.09.2019

İsmail Alper İşoğlu

https://doi.org/10.17798/bitlisfen.530767
Cited By: 1

Öz

Bu çalışmada, yara örtü malzemesi olarak polikaprolakton (PCL), poli(3-hidroksibütirik asit-ko-3-hidroksivalerik asit) (PHBV) ve ağırlıkça farklı oranlarda (100:0, 50:50, 75:25, 0:100) PCL/PHBV karışımları, farklı çözücüler (kloroform (CHCl3), 1,1,1,3,3,3-Hexfluoro-2-propanol (HFIP) ve bunların karışımları) kullanılarak elektroeğirme tekniği ile fibroz yapıda membranlar hazırlanmıştır. Tüm elektroeğrilmiş membranlar Fourier Dönüşümlü Kızılötesi Spektroskopisi (FT-IR), Diferansiyel Taramalı Kalorimetre (DSC) ve Taramalı Elektron Mikroskobu (SEM) ile yapı, morfoloji ve ısıl özellikleri açısından karakterize edilmiştir. Ayrıca, absorbsiyon testi ile sıvı tutma kapasiteleri analiz edilmiştir. Karakterizasyon basamağından sonra, seçilen membranların üzerine insan fibroblast hücreleri ekilmiş, in vitro hücre canlılık ve toksisite, MTT testi ile 24, 48 ve 72. saat için analiz edilmiştir. Membranların üzerine ekilen hücrelerin çoğalması 36, 72 ve 120. saat olmak üzere 3 farklı süre için incelenmiş, sonuçlar SEM ile gösterilmiştir. Elde edilen sonuçlar PCL/PHBV (75:25) karışım ile HFIP çözeltisinde elektroeğrilen membranın yara örtü malzemesi olarak kullanılabileceğini göstermiştir.

Anahtar Kelimeler

PCL PHBV Elektroeğirme Yara Örtü Malzemesi

Kaynakça

  • 1. Kuppan, P., Kirthanashri S.V., Dhakshinamoorthy S., Uma M.K., and Swaminathan S. 2011. Development of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Fibers for Skin Tissue Engineering: Effects of Topography, Mechanical, and Chemical Stimuli, Biomacromolecules, 12 (9): 3156–3165.
  • 2. Zonari, A., Cerqueira M.T., Novikoff, S., Goes A.M., Marques, A.P., Correlo, V.M. and Reis, R.L. 2014. Poly(hydroxybutyrate-co-hydroxyvalerate) Bilayer Skin Tissue Engineering Constructs with Improved Epidermal Rearrangement, Macromolecular Bioscience, 14 (7): 977–990.
  • 3. Du, L., Xu, H.Z., Li, T., Zhang, Y. and Zou, F.Y. 2017. Fabrication of Ascorbyl Palmitate Loaded Poly(caprolactone)/Silver Nanoparticle Embedded Poly(vinyl alcohol) Hybrid Nanofibre Mats as Active Wound Dressings: Via Dual-Spinneret Electrospinning, RSC Advances, 7 (50): 31310–31318.
  • 4. Augustine R., Anto Dominic E., Reju I., Kaimal B., Kalarikkal N., Thomas S. 2015. Electrospun Poly(e-caprolactone)-Based Skin Substitutes: In Vivo Evaluation of Wound Healing and The Mechanism of Cell Proliferation, Journal of Biomedical Materials Research Part B: Applied Biomaterials, 103 (7): 1445–1454.
  • 5. Sahana, T.G., Rekha, P.D. 2018. Biopolymers: Applications in Wound Healing and Skin Tissue Engineering, Molecular Biology Report, 45 (6): 2857-2867.
  • 6. Moura D., Souza M.T., Liverani L., Rella G., Luz G.M., Mano J.F., Boccaccini A.R. 2017. Development of A Bioactive Glass-Polymer Composite for Wound Healing Applications, Materials Science and Engineering C, 76: 224-232.
  • 7. Sundaramurthi D., Krishnan U.M., Sethuraman S. 2014. Epidermal Differentiation of Stem Cells on Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) Nanofibers, Annals of Biomedical Engineering, 42 (12): 2589–2599.
  • 8. Lee J.M., Chae T., Sheikh F.A., Ju H.W., Moon B.M., Park H.J., Park Y.R., Park C.H. 2016. Three Dimensional Poly(ε-caprolactone) and Silk Fibroin Nanocomposite Fibrous Matrix for artificial Dermis, Materials Science and Engineering: C, 68: 758–67.
  • 9. MacEwan M.R., Macewan S., Kovacs T.R., Batts J. 2017. What Makes the Optimal Wound Healing Material? A Review of Current Science and Introduction of a Synthetic Nanofabricated Wound Care Scaffold. 9 (10), 1736-1748.
  • 10. Biazar E. 2017. Application of Polymeric Nanofibers in Medical Designs, Part I: Skin and Eye, International Journal of Polymeric Materials and Polymeric Biomaterials, 66 (10): 521–531.
  • 11. Miguel S.P., Figueira D.R., Simões D., Ribeiro M.P., Coutinho P., Ferreira P., Correia I.J. 2018. Electrospun Polymeric Nanofibres as Wound Dressings: A Review, Colloids Surfaces B Biointerfaces, 169: 60–71.
  • 12. Lv F., Wang J., Xu P., Han Y., Ma H., Xu H., Chen S., Chang J., Ke Q., Liu M., Yi Z., Wu C. 2017. A Conducive Bioceramic/Polymer Composite Biomaterial for Diabetic Wound Healing, Acta Biomaterialia, 60: 128–143.
  • 13. Bölgen N., Menceloǧlu Y.Z., Acatay K., Vargel I., Pişkin E. 2005 In Vitro and In Vivo Degradation of Non-Woven Materials Made of Poly(Ε-Caprolactone) Nanofibers Prepared by Electrospinning Under Different Conditions. Journal of Biomaterials Science, Polymer Edition, 16 (12): 1537–1555.
  • 14. Rather H.A., Thakore R., Singh R., Jhala D., Singh S., Vasita R. Antioxidative Study of Cerium Oxide Nanoparticle Functionalised PCL-Gelatin Electrospun Fibers for Wound Healing Application, Bioactive Materials, 3 (2): 201–211.
  • 15. Yuan J., Geng J., Xing Z., Shim K-J., Han I., Kim J-C., Kang I-K., Shen J. 2015. Novel Wound Dressing Based on Nanofibrous PHBV–Keratin Mats, Journal of Tissue Engineering and Regenerative Medicine, 9: 1027–1035.
  • 16. Tohidi S., Ghaee A., Barzin J. 2016, Preparation and Characterization of Poly(lactic-co-glycolic acid)/Chitosan Electrospun Membrane Containing Amoxicillin-Loaded Halloysite Nanoclay, Polymers for Advanced Technologies, 27: 1020-1028.
  • 17. Dias J.R., Granja P.L., Bártolo P.J. 2016. Advances in Electrospun Skin Substitutes, Progress in Materials Science, 84: 314-334.
  • 18. Zhang W., Ronca S., Mele E. 2017. Electrospun Nanofibres Containing Antimicrobial Plant Extracts, Nanomaterials, 7 (2): 42-59.
  • 19. Patil J.V., Mali S.S., Kamble A.S., Hong C.K., Kim J.H., Patil P.S. 2017. Electrospinning: a Versatile Technique for Making of 1D Growth of Nanostructured Nanofibers and Its Applications: An Experimental Approach. Applied Surface Science, 423: 641–674.
  • 20. Chen S., Liu B., Carlson M.A., Gombart A.F., Reilly D.A., Xie J. 2017. Recent Advances in Electrospun Nanofibers for Wound Healing, Nanomedicine, 12: 1335-1352.
  • 21. Liu M., Duan X., Li Y., Yang D., Long Y. 2017. Electrospun Nano Fibers for Wound Healing, Materials Science and Engineering C, 76: 1413-1423.
  • 22. Mir M., Ali M.N., Barakullah A., Gulzar A., Arshad M, Fatima S., Asad M. 2018. Synthetic Polymeric Biomaterials for Wound Healing: A Review, Progress in Biomaterials, 7 (1): 1-21.
  • 23. Siddiqui N., Asawa S., Birru B., Baadhe R., Rao S. 2018. PCL-Based Composite Scaffold Matrices for Tissue Engineering Applications. Molecular Biotechnology, 60: 506–532.
  • 24. Thanh Tra N., Minh H.H., Nam T.M.H., Thien D.B.T., Hoai, N.T.T., Phuoc, T.V., Thai D.M., Hai, N.D., Toi, V.V., Hiep, N.T. 2018. Optimization and Characterization of Electrospun Polycaprolactone Coated with Gelatin-Silver Nanoparticles for Wound Healing Application, Materials Science & Engineering C, 91: 318-329.
  • 25. Martins A.F., Facchi S.P., da Câmara P.C.F., Camargo S.E.A., Camargo C.H.R., Popat K.C., Kipper M.J. 2018. Novel Poly(ε-Caprolactone)/Amino-Functionalized Tannin Electrospun Membranes as Scaffolds for Tissue Engineering, Journal of Colloid and Interface Science, 525: 21-30.
  • 26. Mutlu G., Calamak S., Ulubayram K., Guven E. 2018. Curcumin-Loaded Electrospun PHBV Nanofibers as Potential Wound-Dressing Material, Journal of Drug Delivery Science and Technology, 43: 185-193.
  • 27. Veleirinho B., Coelho D.S., Dias P.F., Maraschin M., Ribeiro-do-Valle R.M., Lopes-da-Silva J.A. 2012. Nanofibrous Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/Chitosan Scaffolds for Skin Regeneration. International Journal of Biological Macromolecules, 51 (4): 343-350.
  • 28. Shishatskaya E.I., Nikolaeva E.D., Vinogradova O.N., Volova T.G. 2016. Experimental Wound Dressings of Degradable PHA for Skin Defect Repair, Journal of Materials Science: Materials in Medicine, 27 (11): 165.
  • 29. Adeli-Sardou M., Yaghoobi M.M., Torkzadeh-Mahani M., Dodel M. 2019. Controlled Release of Lawsone from Polycaprolactone/Gelatin Electrospun Nano Fibers for Skin Tissue Regeneration, International Journal of Biological Macromolecules, 124: 478-491.
  • 30. Ehterami A., Salehi M., Farzamfar S., Vaez A., Samadian H., Sahrapeyma H., Mirzaii M., Ghorbani S., Goodarzi A. 2018. In Vitro and In Vivo Study of PCL/COLL Wound Dressing Loaded with Insulin-Chitosan Nanoparticles on Cutaneous Wound Healing in Rats Model, International Journal of Biological Macromolecules, 117: 601-609.
  • 31. İşoğlu İ.A., Demirkan C., Şeker M.G., Tuzlakoğlu K., İşoğlu S.D. 2018. Antibacterial Bilayered Skin Patches Made of HPMA and Quaternary Poly(4-Vinyl Pyridine). Fibers and Polymers, 19 (11): 2229-2236.
  • 32. K-Hasuwan P.R., Pavasant P., Supaphol P. 2011. Effect of the Surface Topography of Electrospun Poly(ε-caprolactone)/Poly(3-hydroxybuterate-co-3-hydroxyvalerate) Fibrous Substrates on Cultured Bone Cell Behavior. Langmuir. 27: 10938-10946.
  • 33. Malikmammadov E., Tanir T.E., Kiziltay A., Hasirci V., Hasirci N. 2018. PCL and PCL-Based Materials in Biomedical Applications, Journal of Biomaterials Science, Polymer Edition, 29 (7-9): 863-893.
  • 34. Del Gaudio C., Ercolani E., Nanni F., Bianco A. 2011. Assessment of Poly(ε-caprolactone)/Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Blends Processed by Solvent Casting and Electrospinning, Materials Science and Engineering A, 528 (3): 1764-1772.
  • 35. Chanda A., Adhikari J., Ghosh A., Roy S., Thomas S., Datta P., Saha P. 2018. Electrospun Chitosan/Polycaprolactone-Hyaluronic Acid Bilayered Scaffold for Potential Wound Healing Applications, International Journal of Biological Macromolecules, 116: 774-785.
Toplam 35 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Araştırma Makalesi
Yazarlar

İsmail Alper İşoğlu 0000-0001-6428-4207

Yayımlanma Tarihi 30 Eylül 2019
Gönderilme Tarihi 22 Şubat 2019
Kabul Tarihi 1 Temmuz 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 8 Sayı: 3

Kaynak Göster

IEEE İ. A. İşoğlu, “Yara Örtü Malzemesi Olarak Elektroeğrilmiş PCL/PHBV Membranların Hazırlanması ve Karakterizasyonu”, Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, c. 8, sy. 3, ss. 1029–1044, 2019, doi: 10.17798/bitlisfen.530767.

Cited By

Döndürmeli Kaplama Yöntemi ile Kurkumin Kaplanmış Polikaprolakton Nanolif Yara Örtülerinin Hazırlanması ve in vitro Etkinliğinin İncelenmesi
European Journal of Science and Technology
Didem ŞEN KARAMAN
https://doi.org/10.31590/ejosat.939464
 Kapak Resmi İndir



Bitlis Eren Üniversitesi
Fen Bilimleri Dergisi Editörlüğü

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E-posta: fbe@beu.edu.tr