Research Article
Farklı Su Aktivitesine Sahip Çeşitli Gıdalarda Küf ve Maya Yükünün Belirlenmesi İçin Kullanılan DRBC ve DG18 Besiyerlerinin Etkinliğinin Karşılaştırılması
Abstract
Maya
ve küfler çürüyen bitki materyali, hava ve toprakta oldukça yaygın bir şekilde
bulunmaktadır. Gıda işleme sırasında bu mikroorganizmaların ürüne bulaşması
ciddi kayıplara neden olmaktadır. Bundan dolayı gıdanın florasında bulunan küf
ve mayaların tespit edilmesi önemlidir. Günümüzde bu amaçla yaygın olarak
kullanılan besiyerleri Dichloran Rose Bengal Chloramphenicol (DRBC) agar ve
Dichloran %18 Glycerol (DG18) agardır. Bu çalışmada DRBC agar ve DG18 agar
besiyerlerinin performansı üç farklı grupta su aktivitesine sahip 43 adet gıda
örneğinin küf ve maya florasının belirlenmesinde yayma plak yöntemine göre ekim
yapılarak alınan sayım sonuçları koloni büyüklükleri ve çeşidi
değerlendirilerek bu besiyerlerinin performans karşılaştırılması yapılmıştır. Yapılan
istatistiksel analize göre DRBC agar ve DG18 agar besiyerlerinden tespit edilen
küf-maya sayıları arasında anlamlı bir farklılık olmadığı tespit edilmiştir.
Fakat tüm gıda gruplarına ait petri kaplarının morfolojik olarak
karşılaştırılmasında küf ve maya kolonilerinin büyüklüğü ile küf çeşitliliğinin
DRBC agar besiyerinde daha fazla olduğu görsel olarak tespit edilmiştir.
Keywords
References
- 1. Beuchat L R, Hwang C A (1996). Evaluation of modified dichloran 18% glycerol ( DGl8) agar for enumerating fungi in wheat flour: a collaborative study. International Journal of Food Microbiology 29:161 – 166
- 2. Beuchat L R, Mann D A (2016). Comparison of New and Traditional Culture-Dependent Media for Enumerating Foodborne Yeasts and Molds. Journal o f Food Protection 79:95-111
- 3. Beuchat L R, Fra¨ndberg E, Deak T, Alzamora S M, Chen J, Guerrero S, Lo´pez-Malo A, Ohlsson I, Olsen M, Peinado J M, Schnurer J, de Siloniz M I & Tornai-Lehoczki J (2001). Performance of mycological media in enumerating desiccated food spoilage yeasts: an interlaboratory study. International Journal of Food Microbiology 70:89–96
- 4. Deak T, Chen J, Golden D A, Tapia M S, Tornai-Lehoczki J, Viljoen B C, Wyder M T & Beuchat L R(2001). Comparison of dichloran 18% glycerol DG18/ agar with general purpose mycological media for enumerating food spoilage yeasts. International Journal of Food Microbiology 67: 49–53
- 5. Gourama H, Bullerman L B (1995). Detection of Molds in Foods and Feeds: Potential Rapid and Selective Methods. Journal of Food Protection 58: 1389-1394
- 6. King A D, JR, Hocking A D& Pitt J I (1979). Dichloran-Rose Bengal Medium for Enumeration and Isolation of Molds from Foods. Applied and Environmental Microbiology 37: 959-964
- 7. Taniwaki M H, Da Silva N, Banhe A A& Iamanaka BT (2001). Comparison of Culture Media, Simplate, and Petrifilm for Enumeration of Yeasts and Molds in Food. Journal of Food Protection 64:1592–1596
- 8. Mentese S,Otkun M T & Palaz E (2017). Comparison of dichloran rose bengal chloramphenicol and Sabouraud dextrose agar with cycloheximide and chloramphenicol for airborne mold sampling. Aerobiologia 33(2): 211-219
- 9. Schmidt, S.J. & Fontana, A.J. Jr (2007). Water activity values of select food ingredients and products Appendix E. In: Water Activity in Foods: Fundamentals and Applications (edited by G.V. Barbosa‐Cánovas, A.J. Fontana Jr, S.J. Schmidt & T.P. Labuza). Pp. 407–420. Ames, IA: IFT Press, Blackwell Publishing Professional.
- 10. Fernandez-Salguero, J., Gómez, R., & Carmona, M. A. (1993). Water activity in selected high-moisture foods. Journal of Food Composition and Analysis, 6(4): 364-369.
Year 2018,
Volume: 8 Issue: 2, 206 - 214, 31.12.2018
Abstract
References
- 1. Beuchat L R, Hwang C A (1996). Evaluation of modified dichloran 18% glycerol ( DGl8) agar for enumerating fungi in wheat flour: a collaborative study. International Journal of Food Microbiology 29:161 – 166
- 2. Beuchat L R, Mann D A (2016). Comparison of New and Traditional Culture-Dependent Media for Enumerating Foodborne Yeasts and Molds. Journal o f Food Protection 79:95-111
- 3. Beuchat L R, Fra¨ndberg E, Deak T, Alzamora S M, Chen J, Guerrero S, Lo´pez-Malo A, Ohlsson I, Olsen M, Peinado J M, Schnurer J, de Siloniz M I & Tornai-Lehoczki J (2001). Performance of mycological media in enumerating desiccated food spoilage yeasts: an interlaboratory study. International Journal of Food Microbiology 70:89–96
- 4. Deak T, Chen J, Golden D A, Tapia M S, Tornai-Lehoczki J, Viljoen B C, Wyder M T & Beuchat L R(2001). Comparison of dichloran 18% glycerol DG18/ agar with general purpose mycological media for enumerating food spoilage yeasts. International Journal of Food Microbiology 67: 49–53
- 5. Gourama H, Bullerman L B (1995). Detection of Molds in Foods and Feeds: Potential Rapid and Selective Methods. Journal of Food Protection 58: 1389-1394
- 6. King A D, JR, Hocking A D& Pitt J I (1979). Dichloran-Rose Bengal Medium for Enumeration and Isolation of Molds from Foods. Applied and Environmental Microbiology 37: 959-964
- 7. Taniwaki M H, Da Silva N, Banhe A A& Iamanaka BT (2001). Comparison of Culture Media, Simplate, and Petrifilm for Enumeration of Yeasts and Molds in Food. Journal of Food Protection 64:1592–1596
- 8. Mentese S,Otkun M T & Palaz E (2017). Comparison of dichloran rose bengal chloramphenicol and Sabouraud dextrose agar with cycloheximide and chloramphenicol for airborne mold sampling. Aerobiologia 33(2): 211-219
- 9. Schmidt, S.J. & Fontana, A.J. Jr (2007). Water activity values of select food ingredients and products Appendix E. In: Water Activity in Foods: Fundamentals and Applications (edited by G.V. Barbosa‐Cánovas, A.J. Fontana Jr, S.J. Schmidt & T.P. Labuza). Pp. 407–420. Ames, IA: IFT Press, Blackwell Publishing Professional.
- 10. Fernandez-Salguero, J., Gómez, R., & Carmona, M. A. (1993). Water activity in selected high-moisture foods. Journal of Food Composition and Analysis, 6(4): 364-369.
There are 10 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Review Articles |
| Authors | |
| Publication Date | December 31, 2018 |
| Submission Date | November 1, 2018 |
| Published in Issue | Year 2018 Volume: 8 Issue: 2 |