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Genotoxic And Antigenotoxic Effects Of Corilagin In In Vitro Human Lymphocyte Cultures

Year 2022, Volume: 15 Issue: 3, 819 - 830, 30.12.2022
https://doi.org/10.18185/erzifbed.1148160

Abstract

Bitkiler tarafından ikincil metabolitler olarak sentezlenen doğal bileşiklerin, serbest radikallerin neden olduğu oksidatif strese ve bunların aracılık ettiği genetik hasara karşı koruyucu etkilerinin olduğu birçok araştırmada bildirilmiştir. Bu doğal bileşikler arasında polifenollerin özellikle güçlü antioksidan aktiviteleri olduğu bilinmektedir. Euphorbiaceae, Geraniaceae ve Lythracea familyaları gibi birçok bitki türünde bulunan bir tanen olan korilagin, çok çeşitli farmakolojik etkilere sahiptir. Bu çalışmada, korilaginin güvenli konsantrasyonlarını belirlemek için in vitro insan periferik kan lenfosit kültürlerinde mikroçekirdek (MÇ) ve kromozom anormallikleri (KA) testleri kullanılarak genotoksik/antigenotoksik etkileri araştırıldı. 10-100 µg/ml korilagin hücrelere tek başına ve 0.2 µg/ml MMC ile eş zamanlı olarak uygulandı ve tüm analizler 3 tekrarlı olarak gerçekleştirildi. Analizlerin sonuçlarına göre korilaginin 10, 25, 50 µg/ml uygulama konsantrasyonlarında MÇ ve KA oluşumunda istatistiksel olarak anlamlı bir artışa neden olmadığı hatta MMC uygulaması ile artan MÇ frekansında ve kromozom anormalliklerde istatistiksel olarak önemli oranda azalma sağladığı belirlenmiştir. Diğer yandan bu etkinin konsantrasyona bağlı olduğu ve 100 µg/ml’de DNA hasarını artıcı etki gösterdiği belirlenmiştir. Bu durumun birçok güçlü antioksidanda görülen konsantrasyona bağlı prooksidan aktivite nedeniyle olduğu düşünülmektedir. Çalışma sonuçları korilaginin düşük konsantrasyonlarının MMC gibi genotoksik ajanlar tarafından oluşan genetik hasara karşı antigenotoksik etkisinin olduğunu, kemoterapinin yan etkilerine karşı kullanılabileceğini göstermektedir.

Supporting Institution

Bursa Teknik Üniversitesi

Project Number

190Y013

Thanks

This study was supported by Bursa Technical University Scientific Research Projects Coordinatorship postgraduate thesis project numbered 190Y013

References

  • [1] Aydın, S., Üstün, F. (2007) Tanenler: Kimyasal Yapıları, Farmakolojik Etkileri, Analiz Yöntemleri, İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 33(1), 21-31.
  • [2] Pinto, T., Aires, A., Cosme, F., Bacelar, E., Morais, M. C., Oliveira, I., Gonçalves, B. (2021) Bioactive (Poly) phenols, volatile compounds from vegetables, medicinal and aromatic plants, Foods, 10(1), 106.
  • [3] Hasheminezhad, S. H., Boozari, M., Iranshahi, M., Yazarlu, O., Sahebkar, A., Hasanpour, M., & Iranshahy, M. (2022) A mechanistic insight into the biological activities of urolithins as gut microbial metabolites of ellagitannins., Phytotherapy Research, 36(1), 112-146.
  • [4] Liu, J., Qin, X., Ma, W., Jia, S., Zhang, X., Yang, X., ... & Jin, F. (2021) Corilagin induces apoptosis and autophagy in NRF2‑addicted U251 glioma cell line, Molecular medicine reports, 23(5), 1-10.
  • [5] Yamada, H., Nagao, K., Dokei, K., Kasai, Y., Michihata, N. (2008) Total synthesis of Corilagin, Journal of the American Chemical Society, 130(24), 7566-7567.
  • [6] Ding, Y., Ren, D., Xu, H., Liu, W., Liu, T., Li, L., Li, J., Li, Y., Wen, A. (2017) Antioxidant and pro-angiogenic effects of corilagin in rat cerebral ischemia via Nrf2 activation, Oncotarget, 8(70), 114816.
  • [7] Wang, Z., Guo, Q. Y., Zhang, X. J., Li, X., Li, W. T., Ma, X. T., Ma, L. J. (2014) Corilagin attenuates aerosol bleomycin-induced experimental lung injury, International Journal of Molecular Sciences, 15(6), 9762-9779.
  • [8] Liu, F. C., Chaudry, I. H., & Yu, H. P. (2017) Hepatoprotective effects of corilagin following hemorrhagic shock are through akt-dependent pathway, Shock, 47(3), 346.
  • [9] Yang, L. J., Chen, R. H., Hamdoun, S., Coghi, P., Ng, J. P., Zhang, D. W., ... & Wong, V. K. W. (2021) Corilagin prevents SARS-CoV-2 infection by targeting RBD-ACE2 binding, Phytomedicine, 87, 153591.
  • [10] Li, X., Deng, Y., Zheng, Z., Huang, W., Chen, L., Tong, Q., Ming, Y. (2018) Corilagin, a promising medicinal herbal agent, Biomedicine & Pharmacotherapy, 99, 43-50.
  • [11] Lv, H., Hong, L., Tian, Y., Yin, C., Zhu, C., & Feng, H. (2019) Corilagin alleviates acetaminophen-induced hepatotoxicity via enhancing the AMPK/GSK3β-Nrf2 signaling pathway, Cell Communication and Signaling, 17(1), 1-15.
  • [12] Yisimayili, Z., Guo, X., Liu, H., Xu, Z., Abdulla, R., Aisa, H. A., & Huang, C. (2019) Metabolic profiling analysis of corilagin in vivo and in vitro using high-performance liquid chromatography quadrupole time-of-flight mass spectrometry, Journal of pharmaceutical and biomedical analysis, 165, 251-260.
  • [13] Senobari, Z., Karimi, G., & Jamialahmadi, K. (2022) Ellagitannins, promising pharmacological agents for the treatment of cancer stem cells, Phytotherapy Research, 36(1), 231-242.
  • [14] Labbé, A., & Baudouin, C. (2015) Modulation of Wound Healing: Choice of Antifibrosis Therapies. In Glaucoma (pp. 894-905). WB Saunders.
  • [15] Gad, S. E. (2014). Mitomycin C. 354-356.
  • [16] Schmidt, O. T., & Lademann, R. (1951) Natural tannins. X. Corilagin, another crystalline tannin from dividivi. Liebigs Ann. Chem, 571, 232-237.
  • [17] Heber, D. (2011) Herbal Medicine: Biomolecular and Clinical Aspects. Benzie IFF, Wachtel-Galor S (Ed.), Chapter 10: Pomegranate ellagitannins. CRC Press/Taylor & Francis, England.
  • [18] Prakash, C. V. S., & Prakash, I. (2011) Bioactive chemical constituents from pomegranate (Punica granatum) juice, seed and peel-a review, International Journal of Research in Chemistry and Environment, 1(1), 1-18.
  • [19] Tong, Y., Zhang, G., Li, Y., Xu, J., Yuan, J., Zhang, B., Hu, T., & Song, G. (2018). Corilagin inhibits breast cancer growth via reactive oxygen species‐dependent apoptosis and autophagy, Journal of cellular and molecular medicine, 22(8), 3795-3807.
  • [20] Rencuzogullari, C., Cincin, Z. B., Iplik, E. S., Baran, Y., & Cakmakoglu, B. (2019) Investigation of Potential Anticarcinogenic Effects of Corilagin in Lung Cancer Cells, Clinical and Experimental Health Sciences, 9(3), 228-231.
  • [21] Liu, F. C., Chaudry, I. H., & Yu, H. P. (2017) Hepatoprotective effects of corilagin following hemorrhagic shock are through akt-dependent pathway, Shock (Augusta, Ga.), 47(3), 346.
  • [22] Wu, C., Huang, H., Choi, H. Y., Ma, Y., Zhou, T., Peng, Y., Pang, K., Shu, G., Yang, X. (2021) Anti-esophageal Cancer Effect of Corilagin Extracted from Phmllanthi Fructus via the Mitochondrial and Endoplasmic Reticulum Stress Pathways, Journal of Ethnopharmacology, 269, 113700.
  • [23] Tong, Y., Zhang, G., Li, Y., Xu, J., Yuan, J., Zhang, B., Hu, T., & Song, G. (2018) Corilagin inhibits breast cancer growth via reactive oxygen species‐dependent apoptosis and autophagy, Journal of cellular and molecular medicine, 22(8), 3795-3807.
  • [24] Guo, S., Fu, Y., Xiong, S., & Lv, J. (2017) Corilagin protects the acute lung injury by ameliorating the apoptosis pathway, Biomedicine & Pharmacotherapy, 95, 1743-1748.
  • [25] Tan, S., Su, Y., Huang, L., Deng, S., Yan, G., Yang, X., ... & Cheng, J. (2022) Corilagin attenuates osteoclastic osteolysis by enhancing HO‐1 and inhibiting ROS, Journal of Biochemical and Molecular Toxicology, e23049.
  • [26] Zhang, L., Jia, B., Velu, P., & Wu, H. (2022) Corilagin induces apoptosis and inhibits HMBG1/PI3K/AKT signaling pathways in a rat model of gastric carcinogenesis induced by methylnitronitrosoguanidine, Environmental Toxicology, 37(5), 1222-1230.
  • [27] Huang, J., Lei, Y., Lei, S., & Gong, X. (2022) Cardioprotective effects of corilagin on doxorubicin induced cardiotoxicity via P13K/Akt and NF-κB signaling pathways in a rat model. Toxicology Mechanisms and Methods, 32(2), 79-86.
Year 2022, Volume: 15 Issue: 3, 819 - 830, 30.12.2022
https://doi.org/10.18185/erzifbed.1148160

Abstract

Project Number

190Y013

References

  • [1] Aydın, S., Üstün, F. (2007) Tanenler: Kimyasal Yapıları, Farmakolojik Etkileri, Analiz Yöntemleri, İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 33(1), 21-31.
  • [2] Pinto, T., Aires, A., Cosme, F., Bacelar, E., Morais, M. C., Oliveira, I., Gonçalves, B. (2021) Bioactive (Poly) phenols, volatile compounds from vegetables, medicinal and aromatic plants, Foods, 10(1), 106.
  • [3] Hasheminezhad, S. H., Boozari, M., Iranshahi, M., Yazarlu, O., Sahebkar, A., Hasanpour, M., & Iranshahy, M. (2022) A mechanistic insight into the biological activities of urolithins as gut microbial metabolites of ellagitannins., Phytotherapy Research, 36(1), 112-146.
  • [4] Liu, J., Qin, X., Ma, W., Jia, S., Zhang, X., Yang, X., ... & Jin, F. (2021) Corilagin induces apoptosis and autophagy in NRF2‑addicted U251 glioma cell line, Molecular medicine reports, 23(5), 1-10.
  • [5] Yamada, H., Nagao, K., Dokei, K., Kasai, Y., Michihata, N. (2008) Total synthesis of Corilagin, Journal of the American Chemical Society, 130(24), 7566-7567.
  • [6] Ding, Y., Ren, D., Xu, H., Liu, W., Liu, T., Li, L., Li, J., Li, Y., Wen, A. (2017) Antioxidant and pro-angiogenic effects of corilagin in rat cerebral ischemia via Nrf2 activation, Oncotarget, 8(70), 114816.
  • [7] Wang, Z., Guo, Q. Y., Zhang, X. J., Li, X., Li, W. T., Ma, X. T., Ma, L. J. (2014) Corilagin attenuates aerosol bleomycin-induced experimental lung injury, International Journal of Molecular Sciences, 15(6), 9762-9779.
  • [8] Liu, F. C., Chaudry, I. H., & Yu, H. P. (2017) Hepatoprotective effects of corilagin following hemorrhagic shock are through akt-dependent pathway, Shock, 47(3), 346.
  • [9] Yang, L. J., Chen, R. H., Hamdoun, S., Coghi, P., Ng, J. P., Zhang, D. W., ... & Wong, V. K. W. (2021) Corilagin prevents SARS-CoV-2 infection by targeting RBD-ACE2 binding, Phytomedicine, 87, 153591.
  • [10] Li, X., Deng, Y., Zheng, Z., Huang, W., Chen, L., Tong, Q., Ming, Y. (2018) Corilagin, a promising medicinal herbal agent, Biomedicine & Pharmacotherapy, 99, 43-50.
  • [11] Lv, H., Hong, L., Tian, Y., Yin, C., Zhu, C., & Feng, H. (2019) Corilagin alleviates acetaminophen-induced hepatotoxicity via enhancing the AMPK/GSK3β-Nrf2 signaling pathway, Cell Communication and Signaling, 17(1), 1-15.
  • [12] Yisimayili, Z., Guo, X., Liu, H., Xu, Z., Abdulla, R., Aisa, H. A., & Huang, C. (2019) Metabolic profiling analysis of corilagin in vivo and in vitro using high-performance liquid chromatography quadrupole time-of-flight mass spectrometry, Journal of pharmaceutical and biomedical analysis, 165, 251-260.
  • [13] Senobari, Z., Karimi, G., & Jamialahmadi, K. (2022) Ellagitannins, promising pharmacological agents for the treatment of cancer stem cells, Phytotherapy Research, 36(1), 231-242.
  • [14] Labbé, A., & Baudouin, C. (2015) Modulation of Wound Healing: Choice of Antifibrosis Therapies. In Glaucoma (pp. 894-905). WB Saunders.
  • [15] Gad, S. E. (2014). Mitomycin C. 354-356.
  • [16] Schmidt, O. T., & Lademann, R. (1951) Natural tannins. X. Corilagin, another crystalline tannin from dividivi. Liebigs Ann. Chem, 571, 232-237.
  • [17] Heber, D. (2011) Herbal Medicine: Biomolecular and Clinical Aspects. Benzie IFF, Wachtel-Galor S (Ed.), Chapter 10: Pomegranate ellagitannins. CRC Press/Taylor & Francis, England.
  • [18] Prakash, C. V. S., & Prakash, I. (2011) Bioactive chemical constituents from pomegranate (Punica granatum) juice, seed and peel-a review, International Journal of Research in Chemistry and Environment, 1(1), 1-18.
  • [19] Tong, Y., Zhang, G., Li, Y., Xu, J., Yuan, J., Zhang, B., Hu, T., & Song, G. (2018). Corilagin inhibits breast cancer growth via reactive oxygen species‐dependent apoptosis and autophagy, Journal of cellular and molecular medicine, 22(8), 3795-3807.
  • [20] Rencuzogullari, C., Cincin, Z. B., Iplik, E. S., Baran, Y., & Cakmakoglu, B. (2019) Investigation of Potential Anticarcinogenic Effects of Corilagin in Lung Cancer Cells, Clinical and Experimental Health Sciences, 9(3), 228-231.
  • [21] Liu, F. C., Chaudry, I. H., & Yu, H. P. (2017) Hepatoprotective effects of corilagin following hemorrhagic shock are through akt-dependent pathway, Shock (Augusta, Ga.), 47(3), 346.
  • [22] Wu, C., Huang, H., Choi, H. Y., Ma, Y., Zhou, T., Peng, Y., Pang, K., Shu, G., Yang, X. (2021) Anti-esophageal Cancer Effect of Corilagin Extracted from Phmllanthi Fructus via the Mitochondrial and Endoplasmic Reticulum Stress Pathways, Journal of Ethnopharmacology, 269, 113700.
  • [23] Tong, Y., Zhang, G., Li, Y., Xu, J., Yuan, J., Zhang, B., Hu, T., & Song, G. (2018) Corilagin inhibits breast cancer growth via reactive oxygen species‐dependent apoptosis and autophagy, Journal of cellular and molecular medicine, 22(8), 3795-3807.
  • [24] Guo, S., Fu, Y., Xiong, S., & Lv, J. (2017) Corilagin protects the acute lung injury by ameliorating the apoptosis pathway, Biomedicine & Pharmacotherapy, 95, 1743-1748.
  • [25] Tan, S., Su, Y., Huang, L., Deng, S., Yan, G., Yang, X., ... & Cheng, J. (2022) Corilagin attenuates osteoclastic osteolysis by enhancing HO‐1 and inhibiting ROS, Journal of Biochemical and Molecular Toxicology, e23049.
  • [26] Zhang, L., Jia, B., Velu, P., & Wu, H. (2022) Corilagin induces apoptosis and inhibits HMBG1/PI3K/AKT signaling pathways in a rat model of gastric carcinogenesis induced by methylnitronitrosoguanidine, Environmental Toxicology, 37(5), 1222-1230.
  • [27] Huang, J., Lei, Y., Lei, S., & Gong, X. (2022) Cardioprotective effects of corilagin on doxorubicin induced cardiotoxicity via P13K/Akt and NF-κB signaling pathways in a rat model. Toxicology Mechanisms and Methods, 32(2), 79-86.
There are 27 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Makaleler
Authors

Elif Turan 0000-0003-1836-6636

Gökçe Taner 0000-0002-0290-1166

Project Number 190Y013
Early Pub Date December 27, 2022
Publication Date December 30, 2022
Published in Issue Year 2022 Volume: 15 Issue: 3

Cite

APA Turan, E., & Taner, G. (2022). Genotoxic And Antigenotoxic Effects Of Corilagin In In Vitro Human Lymphocyte Cultures. Erzincan University Journal of Science and Technology, 15(3), 819-830. https://doi.org/10.18185/erzifbed.1148160