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Serological Investigation of Bovine Enterovirus in Calves in Konya Province

Year 2024, Volume: 21 Issue: 1, 43 - 49, 01.04.2024

Hasan Sercan Palancı Oğuzhan Avcı Irmak Dik Hatice Pelin Aslım Rüveyde Gülbahçe Oya Bulut

https://doi.org/10.32707/ercivet.1455296

Abstract

Bovine enterovirus (BEV) infection is a common viral disease in cattle. Although the infection is often subclinical, it is among the possible causes of gastroenteritis. BEV infections have also been associated with respiratory and reproductive system disorders and signs of diarrhea. BEV is divided into two serotypes, enterovirus serotype E (EV-E) and enterovirus serotype F (EV-F). This study aims to determine the seroprevalence of EV-E in calves in Konya province. For this purpose, 504 BEV unvaccinated calf blood serum samples from the Selcuk University Veterinary Faculty Virology Department laboratory were used. A serum neutralization test (SNT) was used to determine EV-E seroprevalence. 342 (67.85%) samples were found to be seropositive, and 162 (32.14%) were seronegative. In addition, as the serum neutralization 50 (SN50) antibody titers of seropositive animals were examined, the titers determined as 1/10, 1/15 and 1/20. Among the results we obtained, the highest antibody titers were defined as 1/80 and 1/120; the total number of animals with these values was In conclusion, bovine enteroviruses are an infection of importance for cattle breeding. Therefore, necessary precautions must be taken to protection infection. It is thought that the results of the present study will provide important data for future studies.

Keywords

Antibody bovine enterovirus serum neutralization test

References

  • Acar A, Gür S. Seroprevalence of bovine enterovirus type 1 (BEV1) in goats in Turkey. J Anim Vet Adv 2009; 8(6): 1075-8.
  • Alkan F, Özkul A, Karaoğlu MT, Bilge S, Akça Y, Bur-gu I, Yeşilbağ K, Oğuzoğlu TÇ. Seroepidemiology of viral respiratory tract infections in cattle. Ankara Univ Vet Fak Derg 1997; 44(1): 1-8.
  • Beato MS, Marcacci M, Schiavon E, Bertocchi L, Di Domenico M, Peserico A, Mion M, Zaccaria G, Cavicchio L, Mangone I, Soranzo E, Patavino C, Camma C, Lorusso A. Identification and genetic characterization of bovine enterovirus by combination of two next generation sequencing platforms. J Virol Methods 2018; 260: 21-5.
  • Birdane FM, Gür S. Precolostral evaluation of Bovine Enterovirus Type 1 (BEV1) infection in a dairy herd. Vet Arhiv 2019; 89(1): 1-10.
  • Blas-Machado U, Saliki JT, Boileau MJ, Goens SD, Caseltine SL, Duffy JC, Welsh RD. Fatal ulcerative and hemorrhagic typhlocolitis in a pregnant heifer associated with natural bovine enterovirus type-1 infection. Vet Pathol 2007; 44(1):110-5.
  • Blas-Machado U, Saliki JT, Sánchez S, Brown C, Zhang J, Keys D, Woolums A, Harvey SB. Pathogenesis of a bovine enterovirus-1 isolate in experimentally infected calves. Vet Pathol 2011; 48(6): 1075-84.
  • Candido M, Almeida-Queiroz SR, Buzinaro MG, Livo-nesi MC, Fernandes AM, Sousa RLM. Detection and molecular characterisation of bovine Entero-virus in Brazil: four decades since the first report. Epidemiol Infect 2019; 147(e126): 1-2.
  • Castells M, Colina R. Viral Enteritis in Cattle: To Well Known Viruses and Beyond. Microbiol Res 2021; 12(3): 663-72.
  • Christianson WT. Stillbirths, mummies, abortions, and early embryonic death. Vet Clin of North Am Food Anim Pract 1992; 8 (3): 623-9.
  • Erol N, Gür S, Koç BT, Yavru S. A serological investigation of Bovine enterovirus-1, Bovine herpesvirus-1, Bovine viral diarrhea virus, and Parainflu-enza-3 infections in camels in Western Turkey. Vet Ital 2020; 56(4): 257-62.
  • Gras CK, Demoliner M, Eisen AKA, Spilki FR, Henzel A. Seroprevalence of bovine adenovirus and enterovirus antibodies reveals different infection dynamics in cattle herds. Acta Sci Vet 2017; 45(1): 1-6.
  • Gür S, Akça Y, Burgu I. Serological investigation of bovine enterovirus type-1 in buffaloes in Turkey. Ankara Univ Vet Fak Derg 2006; 53(3): 191-4.
  • Gür S, Yapkiç O, Yilmaz A. Serological survey of bovine enterovirus type 1 in different mammalian species in Turkey. Zoonoses Public Health 2008; 55(2): 106-11.
  • Gür S, Gürçay M, Seyrek A. A study regarding bovine enterovirus type 1 infection in domestic animals and humans. J Vet Med Sci 2019; 81(12): 1824-8.
  • Hamblin C, Knowles NJ, Hedger RS. Isolation and identification of bovid enteroviruses from free-living wild animals in Botswana. Vet Rec 1985; 116(9): 238-9.
  • Jain NC, Batra SK. Isolation and characterization of ovine enteroviruses. Indian J Virol 1985; 1(1): 17-25.
  • Kärber G. Beitrag zur kollektiven Behandlung phar-makologischer Reihenversuche. Naunyn Schmiedebergs Arch Pharmacol 1931; 162(4): 480-3.
  • Li Y, Chang J, Wang Q, Yu L. Isolation of two Chinese bovine enteroviruses and sequence analysis of their complete genomes. Arch Virol 2012; 157(12): 2369-5.
  • Luo Y, Liu H, Zou Y, Qiao C, Su Y, Zhu X, Zhang G, Huang W, Qin Y, Pan Y, Huang W. Molecular epidemiology of bovine enteroviruses and genome characterization of two novel bovine entero-virus strains in Guangxi, China. Microbiol Spectr 2023; 11(2): e03785-22.
  • Mc Clenahan SD, Scherba G, Borst L, Fredrickson RL, Krause PR, Uhlenhaut C. Discovery of a bo-vine enterovirus in alpaca. PLoS One 2013; 8(8): e68777.
  • Mosena AC, da Silva MS, Gularte JS, Demoliner M, Girardi V, Flores EF, Weiblen R, Spilki FR. Genome sequence of a Brazilian bovine enterovirus. Microbiol Resour Announc 2022; 11(2): e01200-21.
  • Nollens HH, Rivera R, Palacios G, Wellehan JFX, Saliki JT, Caseltine SL, Smith CR, Jensen ED, Hui J, Lipkin WI, Yochem PK, Wells RS, St Leger J, Venn-Watson S. New recognition of enterovirus infections in bottlenose dolphins (Tursiops trunca-tus). Vet Microbiol 2009; 139(1-2): 170-5.
  • Park JH, Kim SM, Bang MW, Lee KN, Ko YJ, Lee HS, Shim HS, Cho IS. Existence of antibodies against bovine enterovirus in humans and various animals in Korea. Korean J Vet Res 2009; 49(3): 237-42.
  • Puntel M, Fondevila NA, Viera JB, O’Donnell VK, Marcovecchio JF, Carrillo BJ, Schudel AA. Sero-logical survey of viral antibodies in llamas (Lama glama) in Argentina. Zentralbl Veterinarmed B 1999; 46(3): 157-61.
  • Rao CD. Enteroviruses in gastrointestinal diseases. Rev Med Virol 2021;31(1):1-12.
  • Ren X, Zhang S, Gao X, Guo X, Xin T, Zhu H, Jia H, Hou S. Experimental immunization of mice with a recombinant bovine enterovirus vaccine expressing BVDV E0 protein elicits a long-lasting serologic response. Virol J 2020; 17(1): 1-10.
  • Solomon T, Lewthwaite P, Perera D, Cardosa MJ, McMinn P, Ooi MH. Virology, epidemiology, pathogenesis, and control of enterovirus 71. Lancet Infect Dis 2010; 10(11): 778-80.
  • Spearman C. The method of right and wrong cases (constant stimuli) without Gauss's formulae. Br J of Psychol 1908; 2(3): 227-32.
  • Urakawa T, Shingu M. Studies on the classification of bovine enteroviruses. Microbiol Immunol 1987; 31(8):771-8.
  • Wang X, Peng W, Ren J, Hu Z, Xu J, Lou Z, Li X, Yin W, Shen X, Porta C. A sensor-adaptor mechanism for enterovirus uncoating from structures of EV71. Nat Struct Mol Biol 2012; 19(4): 424-9.
  • Zell R, Delwart E, Gorbalenya AE, Hovi T, King AMQ, Knowles NJ, Lindberg AM, Pallansch MA, Pal-menberg AC, Reuter G, Simmonds P, Skern T, Stanway G, Yamashita T. ICTV Virus Taxonomy Profile: Picornaviridae. J Gen Virol; 2017; 98(10): 2421-2.
  • Zhang H, Liu H, Bao J, Guo Y, Peng T, Zhou P, Zhang W, Ma B, Wang J, Gao M. Characterization of an Enterovirus species E isolated from naturally infected bovine in China. Virus Res 2014; 191: 101-7.
  • Zheng T. Characterisation of two enteroviruses isolated from Australian brushtail possums (Trichosurus vulpecula) in New Zealand. Arch Virol 2007; 152(1): 191-8.
  • Zheng T, Hamel KL, Buddle BM. A serological survey of the prevalence of antibodies against enteroviruses in brushtail possums (Trichosurus vulpecula) in New Zealand: Enteroviruses have a limited distribution in brushtail possums. N Z Vet J 2010; 58(1): 23-8.

Konya Bölgesi’ndeki Buzağılarda Bovine Enterovirus’un Serolojik Araştırılması

Year 2024, Volume: 21 Issue: 1, 43 - 49, 01.04.2024

Hasan Sercan Palancı Oğuzhan Avcı Irmak Dik Hatice Pelin Aslım Rüveyde Gülbahçe Oya Bulut

https://doi.org/10.32707/ercivet.1455296

Abstract

Bovine enterovirus (BEV) sığır popülasyonunda yaygın olarak gözlenen viral bir enfeksiyondur. Enfeksiyon daha sıklıkla subklinik olmasına rağmen, gastroenterit hastalığının olası nedenleri arasında yer almaktadır. BEV enfeksiyon-ları aynı zamanda solunum ve üreme sistemi bozuklukları ve ishal bulguları ile de ilişkilendirilmektedir. BEV, enterovi-rus serotip E (EV-E) ve enterovirus serotip F (EV-F) olmak üzere iki serotipe ayrılır. Bu çalışma Konya bölgesinde buzağılarda EV-E seroprevalansını tespit etmek amacı ile yapıldı. Bu amaçla Selçuk Üniversitesi Veteriner Fakültesi Viroloji Anabilim Dalı laboratuvarında bulunan 504 adet BEV yönünden aşısız buzağı kan serum numuneleri kullanıldı. Kan serum örneklerine EV-E seroprevelansının belirlenmesi için serum nötralizasyon testi (SNT) uygulandı. 342 (%67.85) örnek seropozitif 162 (%32.14) örnek ise seronegatif tespit edildi. Ayrıca seropozitif hayvanların Serum Nötrali-zasyon 50 (SN50) titreleri incelendiğinde nötralize edici antikor titreleri 1/10, 1/15 ve 1/20 olarak tespit edildi. Sonuçlar arasında en yüksek antikor titreleri1/80 ve 1/120 olarak tanımlandı ve bu değerlere sahip total hayvan sayısı ise 18 olarak tespit edildi. Sonuç olarak, sığır enterovirusları sığır yetiştiriciliği için önem arz eden bir enfeksiyondur. Bu nedenle enfeksiyondan korunmak için gerekli önlemlerin alınması gerekmektedir. Mevcut çalışmadan elde edilen sonuçların gelecekte yapılması planlanan çalışmalar için önemli veriler sağlayacağı düşünülmektedir.

Keywords

Antikor serum nötralizasyon testi sığır enterovirus

References

  • Acar A, Gür S. Seroprevalence of bovine enterovirus type 1 (BEV1) in goats in Turkey. J Anim Vet Adv 2009; 8(6): 1075-8.
  • Alkan F, Özkul A, Karaoğlu MT, Bilge S, Akça Y, Bur-gu I, Yeşilbağ K, Oğuzoğlu TÇ. Seroepidemiology of viral respiratory tract infections in cattle. Ankara Univ Vet Fak Derg 1997; 44(1): 1-8.
  • Beato MS, Marcacci M, Schiavon E, Bertocchi L, Di Domenico M, Peserico A, Mion M, Zaccaria G, Cavicchio L, Mangone I, Soranzo E, Patavino C, Camma C, Lorusso A. Identification and genetic characterization of bovine enterovirus by combination of two next generation sequencing platforms. J Virol Methods 2018; 260: 21-5.
  • Birdane FM, Gür S. Precolostral evaluation of Bovine Enterovirus Type 1 (BEV1) infection in a dairy herd. Vet Arhiv 2019; 89(1): 1-10.
  • Blas-Machado U, Saliki JT, Boileau MJ, Goens SD, Caseltine SL, Duffy JC, Welsh RD. Fatal ulcerative and hemorrhagic typhlocolitis in a pregnant heifer associated with natural bovine enterovirus type-1 infection. Vet Pathol 2007; 44(1):110-5.
  • Blas-Machado U, Saliki JT, Sánchez S, Brown C, Zhang J, Keys D, Woolums A, Harvey SB. Pathogenesis of a bovine enterovirus-1 isolate in experimentally infected calves. Vet Pathol 2011; 48(6): 1075-84.
  • Candido M, Almeida-Queiroz SR, Buzinaro MG, Livo-nesi MC, Fernandes AM, Sousa RLM. Detection and molecular characterisation of bovine Entero-virus in Brazil: four decades since the first report. Epidemiol Infect 2019; 147(e126): 1-2.
  • Castells M, Colina R. Viral Enteritis in Cattle: To Well Known Viruses and Beyond. Microbiol Res 2021; 12(3): 663-72.
  • Christianson WT. Stillbirths, mummies, abortions, and early embryonic death. Vet Clin of North Am Food Anim Pract 1992; 8 (3): 623-9.
  • Erol N, Gür S, Koç BT, Yavru S. A serological investigation of Bovine enterovirus-1, Bovine herpesvirus-1, Bovine viral diarrhea virus, and Parainflu-enza-3 infections in camels in Western Turkey. Vet Ital 2020; 56(4): 257-62.
  • Gras CK, Demoliner M, Eisen AKA, Spilki FR, Henzel A. Seroprevalence of bovine adenovirus and enterovirus antibodies reveals different infection dynamics in cattle herds. Acta Sci Vet 2017; 45(1): 1-6.
  • Gür S, Akça Y, Burgu I. Serological investigation of bovine enterovirus type-1 in buffaloes in Turkey. Ankara Univ Vet Fak Derg 2006; 53(3): 191-4.
  • Gür S, Yapkiç O, Yilmaz A. Serological survey of bovine enterovirus type 1 in different mammalian species in Turkey. Zoonoses Public Health 2008; 55(2): 106-11.
  • Gür S, Gürçay M, Seyrek A. A study regarding bovine enterovirus type 1 infection in domestic animals and humans. J Vet Med Sci 2019; 81(12): 1824-8.
  • Hamblin C, Knowles NJ, Hedger RS. Isolation and identification of bovid enteroviruses from free-living wild animals in Botswana. Vet Rec 1985; 116(9): 238-9.
  • Jain NC, Batra SK. Isolation and characterization of ovine enteroviruses. Indian J Virol 1985; 1(1): 17-25.
  • Kärber G. Beitrag zur kollektiven Behandlung phar-makologischer Reihenversuche. Naunyn Schmiedebergs Arch Pharmacol 1931; 162(4): 480-3.
  • Li Y, Chang J, Wang Q, Yu L. Isolation of two Chinese bovine enteroviruses and sequence analysis of their complete genomes. Arch Virol 2012; 157(12): 2369-5.
  • Luo Y, Liu H, Zou Y, Qiao C, Su Y, Zhu X, Zhang G, Huang W, Qin Y, Pan Y, Huang W. Molecular epidemiology of bovine enteroviruses and genome characterization of two novel bovine entero-virus strains in Guangxi, China. Microbiol Spectr 2023; 11(2): e03785-22.
  • Mc Clenahan SD, Scherba G, Borst L, Fredrickson RL, Krause PR, Uhlenhaut C. Discovery of a bo-vine enterovirus in alpaca. PLoS One 2013; 8(8): e68777.
  • Mosena AC, da Silva MS, Gularte JS, Demoliner M, Girardi V, Flores EF, Weiblen R, Spilki FR. Genome sequence of a Brazilian bovine enterovirus. Microbiol Resour Announc 2022; 11(2): e01200-21.
  • Nollens HH, Rivera R, Palacios G, Wellehan JFX, Saliki JT, Caseltine SL, Smith CR, Jensen ED, Hui J, Lipkin WI, Yochem PK, Wells RS, St Leger J, Venn-Watson S. New recognition of enterovirus infections in bottlenose dolphins (Tursiops trunca-tus). Vet Microbiol 2009; 139(1-2): 170-5.
  • Park JH, Kim SM, Bang MW, Lee KN, Ko YJ, Lee HS, Shim HS, Cho IS. Existence of antibodies against bovine enterovirus in humans and various animals in Korea. Korean J Vet Res 2009; 49(3): 237-42.
  • Puntel M, Fondevila NA, Viera JB, O’Donnell VK, Marcovecchio JF, Carrillo BJ, Schudel AA. Sero-logical survey of viral antibodies in llamas (Lama glama) in Argentina. Zentralbl Veterinarmed B 1999; 46(3): 157-61.
  • Rao CD. Enteroviruses in gastrointestinal diseases. Rev Med Virol 2021;31(1):1-12.
  • Ren X, Zhang S, Gao X, Guo X, Xin T, Zhu H, Jia H, Hou S. Experimental immunization of mice with a recombinant bovine enterovirus vaccine expressing BVDV E0 protein elicits a long-lasting serologic response. Virol J 2020; 17(1): 1-10.
  • Solomon T, Lewthwaite P, Perera D, Cardosa MJ, McMinn P, Ooi MH. Virology, epidemiology, pathogenesis, and control of enterovirus 71. Lancet Infect Dis 2010; 10(11): 778-80.
  • Spearman C. The method of right and wrong cases (constant stimuli) without Gauss's formulae. Br J of Psychol 1908; 2(3): 227-32.
  • Urakawa T, Shingu M. Studies on the classification of bovine enteroviruses. Microbiol Immunol 1987; 31(8):771-8.
  • Wang X, Peng W, Ren J, Hu Z, Xu J, Lou Z, Li X, Yin W, Shen X, Porta C. A sensor-adaptor mechanism for enterovirus uncoating from structures of EV71. Nat Struct Mol Biol 2012; 19(4): 424-9.
  • Zell R, Delwart E, Gorbalenya AE, Hovi T, King AMQ, Knowles NJ, Lindberg AM, Pallansch MA, Pal-menberg AC, Reuter G, Simmonds P, Skern T, Stanway G, Yamashita T. ICTV Virus Taxonomy Profile: Picornaviridae. J Gen Virol; 2017; 98(10): 2421-2.
  • Zhang H, Liu H, Bao J, Guo Y, Peng T, Zhou P, Zhang W, Ma B, Wang J, Gao M. Characterization of an Enterovirus species E isolated from naturally infected bovine in China. Virus Res 2014; 191: 101-7.
  • Zheng T. Characterisation of two enteroviruses isolated from Australian brushtail possums (Trichosurus vulpecula) in New Zealand. Arch Virol 2007; 152(1): 191-8.
  • Zheng T, Hamel KL, Buddle BM. A serological survey of the prevalence of antibodies against enteroviruses in brushtail possums (Trichosurus vulpecula) in New Zealand: Enteroviruses have a limited distribution in brushtail possums. N Z Vet J 2010; 58(1): 23-8.
There are 34 citations in total.

Details

Primary Language English
Subjects Veterinary Virology
Journal Section Articles
Authors

Hasan Sercan Palancı 0000-0001-5408-9176

Oğuzhan Avcı 0000-0001-9299-4695

Irmak Dik 0000-0003-2516-9489

Hatice Pelin Aslım 0000-0001-9160-1255

Rüveyde Gülbahçe 0000-0002-3665-4642

Oya Bulut 0000-0002-2407-7390

Publication Date April 1, 2024
Submission Date August 11, 2023
Acceptance Date November 17, 2023
Published in Issue Year 2024 Volume: 21 Issue: 1

Cite

APA Palancı, H. S., Avcı, O., Dik, I., Aslım, H. P., et al. (2024). Serological Investigation of Bovine Enterovirus in Calves in Konya Province. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 21(1), 43-49. https://doi.org/10.32707/ercivet.1455296
AMA Palancı HS, Avcı O, Dik I, Aslım HP, Gülbahçe R, Bulut O. Serological Investigation of Bovine Enterovirus in Calves in Konya Province. Erciyes Üniv Vet Fak Derg. April 2024;21(1):43-49. doi:10.32707/ercivet.1455296
Chicago Palancı, Hasan Sercan, Oğuzhan Avcı, Irmak Dik, Hatice Pelin Aslım, Rüveyde Gülbahçe, and Oya Bulut. “Serological Investigation of Bovine Enterovirus in Calves in Konya Province”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 21, no. 1 (April 2024): 43-49. https://doi.org/10.32707/ercivet.1455296.
EndNote Palancı HS, Avcı O, Dik I, Aslım HP, Gülbahçe R, Bulut O (April 1, 2024) Serological Investigation of Bovine Enterovirus in Calves in Konya Province. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 21 1 43–49.
IEEE H. S. Palancı, O. Avcı, I. Dik, H. P. Aslım, R. Gülbahçe, and O. Bulut, “Serological Investigation of Bovine Enterovirus in Calves in Konya Province”, Erciyes Üniv Vet Fak Derg, vol. 21, no. 1, pp. 43–49, 2024, doi: 10.32707/ercivet.1455296.
ISNAD Palancı, Hasan Sercan et al. “Serological Investigation of Bovine Enterovirus in Calves in Konya Province”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 21/1 (April 2024), 43-49. https://doi.org/10.32707/ercivet.1455296.
JAMA Palancı HS, Avcı O, Dik I, Aslım HP, Gülbahçe R, Bulut O. Serological Investigation of Bovine Enterovirus in Calves in Konya Province. Erciyes Üniv Vet Fak Derg. 2024;21:43–49.
MLA Palancı, Hasan Sercan et al. “Serological Investigation of Bovine Enterovirus in Calves in Konya Province”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, vol. 21, no. 1, 2024, pp. 43-49, doi:10.32707/ercivet.1455296.
Vancouver Palancı HS, Avcı O, Dik I, Aslım HP, Gülbahçe R, Bulut O. Serological Investigation of Bovine Enterovirus in Calves in Konya Province. Erciyes Üniv Vet Fak Derg. 2024;21(1):43-9.
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